Devitrification Protocol with Sage
The process consists of 4 steps to be followed at 37ºC:
1Place the Cryotop with the oocytes into Medium 1, where the oocytes will remain for 1 minute.
2Transfer from Medium 1 to Medium 2 (using the minimum possible medium), where they will remain for 3 minutes.
3Transfer from Medium 2 to Medium 3 (using the minimum possible medium), where they will remain for 5 minutes.
4Transfer from Medium 3 to Medium 4 (using the minimum possible medium), where they will remain for 5 minutes.
Finally, transfer the oocytes from Medium 4 to their culture plate, which is then placed in the incubator.
