Devitrification Protocol with Sage
The process consists of 4 steps to be followed at 37ºC:
1Place the Cryotop with the oocytes into Medium 1, where the oocytes will remain for 1 minute.
2Transfer from Medium 1 to Medium 2 (using the minimum possible medium), where they will remain for 3 minutes.
3Transfer from Medium 2 to Medium 3 (using the minimum possible medium), where they will remain for 5 minutes.
4Transfer from Medium 3 to Medium 4 (using the minimum possible medium), where they will remain for 5 minutes.
Finally, transfer the oocytes from Medium 4 to their culture plate, which is then placed in the incubator.
The Devitrification Protocol with Sage is a trusted method used by fertility laboratories to safely and effectively warm vitrified oocytes. Designed to support optimal oocyte survival, this step-by-step protocol minimizes osmotic stress and preserves cellular integrity throughout the warming process. Sage media offers precise composition and quality control, ensuring consistent results and high success rates in assisted reproduction. The protocol involves a carefully timed sequence of warming and dilution steps, using solutions that gradually rehydrate the oocyte while maintaining ideal pH and temperature conditions. Thanks to its ease of use, reproducibility, and proven efficiency, Sage is widely adopted in top fertility centers worldwide. Thanks to its ease of use, reproducibility, and proven efficiency, Sage is widely adopted in top fertility centers worldwide. Whether you’re working with egg donation programs, IVF patients, or fertility preservation cases, following the Sage devitrification protocol helps ensure reliable outcomes. Proper training and adherence to each stage of the process are key to maximizing oocyte viability and improving fertilization potential. Whether you’re working with egg donation programs or IVF patients, following the Sage devitrification protocol helps ensure reliable outcomes. Proper training and adherence to each stage of the process are key to maximizing oocyte viability and improving fertilization potential. Learn the Devitrification Protocol with Sage step by step and elevate your lab’s performance with confidence and precision.