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Devitrification Protocol with Omni Vitrolife
Consists of 4 steps to follow:
1Place the Cryotop with the oocytes in Medium 1, where the oocytes will remain for 1 min (at 37 ºC).
2Transfer from Medium 1 to Medium 2 (using the minimum possible medium), where they will remain for 3 min (at 37 ºC).
3Transfer from Medium 2 to Medium 3 (using the minimum possible medium), where they will remain for 5 min (at 37 ºC).
4Transfer from Medium 3 to Medium 4 (using the minimum possible medium), where they will remain for 10 min (at 37 ºC).
Finally, transfer the oocytes from Medium 4 to their culture plate, which is then placed in the incubator.
Learn the Devitrification Protocol with Omni Vitrolife step by step to ensure optimal oocyte survival and high success rates in assisted reproduction. This well-established protocol is trusted by fertility laboratories worldwide for its reliability, ease of use, and consistent outcomes during the warming process of vitrified oocytes. Omni Vitrolife’s devitrification media are specially formulated to support cell integrity, providing a controlled environment that allows for efficient rehydration and recovery of the oocytes. The protocol follows a precise sequence of warming and dilution steps, ensuring minimal osmotic shock and maximizing the potential for successful fertilization. Each phase of the protocol has been carefully optimized to support embryologists in achieving repeatable and high-quality results. From the warming solution to the final rinse, every step plays a crucial role in protecting the delicate structure of the oocytes. Whether you are implementing this protocol for the first time or looking to perfect your technique, the Omni Vitrolife method offers a comprehensive and user-friendly solution for modern IVF laboratories. Access our expert training resources, protocol breakdowns, and video demonstrations to enhance your knowledge and boost your lab’s performance with Vitrolife’s advanced technology.
