Devitrification Protocol with Kitazato
Preparation
Liquid nitrogen
Remove the lid
Warming
TS 1-4ml
1 min. 37 ºC
Ready to transfer to the culture plate
ULTRA-FAST WARMING PROTOCOL – KITAZATO
The ultra-fast warming procedure is a technique that optimizes the thawing of oocytes and embryos in just 1 minute.
1Heat the TS medium in an appropriate dish until it
reaches a temperature of 37°C (heat without CO₂).
2Remove the external cover of the Cryotop under liquid nitrogen.
3Place the Cryotop with the vitrified sample into the preheated dish at 37°C with 4 mL of TS medium and leave it for 1 minute.
4After 1 minute, transfer the thawed sample to its corresponding pre-gassed culture plate and maintain it at 37°C in an incubator.
